Bruno M. Colombo is full professor in Cell Biology (Université d’Evry-val d’Essonne, Evry, France).
Born in Milan (Italy) in 1963, his research activities are focused on Cancer Research since 1987. He obtained a doctoral thesis in Molecular and Cell Biology in Milan (1989), where he worked as a post-doc fellow until 1995. He participated in studies on the genetic mechanisms involved in tumor formation and progression. He also participated in the development of some gene therapy strategies.
Since 1995, he is working in Paris (holding a PhD in Immunology) on the relationship between tumor, immune cells and angiogenesis. He was involved in the development of strategies of cellular immunotherapy.
He is now working to understand the mechanisms responsible for the suppression of anticancer immune responses.

Inside I3, Bruno M. Colombo is a member of the A2C team, co-manager of the transversal activity "Training and Dissemination" and the administrator of this web site. E-mail: bruno.colombo@upmc.fr

Publications
Genetic mechanisms in tumor formation and progression:
-T.A. Dragani, G. Manenti, M.R.M. Sacchi, B.M. Colombo and G. Della Porta. Major urinary protein as a negative tumor marker in mouse hepatocarcinogenesis. Molecular Carcinogenesis 2, 355-360; 1989.
- T.A. Dragani, G. Manenti, M.R.M. Sacchi, B.M. Colombo and G. Della Porta. Alterations in gene expression in mouse hepatocarcinogenesis. In: "Biologically-Based Methods for Cancer Risk Assessment". Ed. Curtis C. Travis, NATO ASI Series, Plenum Publishing Corporation, pp. 193-207; 1989.
- T.A. Dragani, G. Manenti, B.M. Colombo and G. Della Porta. Genetic susceptibility to murine hepatocarcinogenesis. In: "Chemical Carcinogenesis 2". Eds. A. Columbano, F. Feo, R. Pascale, P. Pani. Plenum Press, New York, pp. 165-176; 1991.
- T.A. Dragani, G. Manenti, B.M. Colombo, F.S. Falvella, M. Gariboldi, M.A. Pierotti and G. Della Porta. Incidence of mutations at codon 61 of the Ha-ras gene in liver tumors of mice genetically susceptible and resistant to hepatocarcinogenesis. Oncogene 6, 333-338; 1991.
- B.M. Colombo, L. Falquerho, G. Manenti, T.A Dragani and A. Le Cam. Expression of the pp63 gene encoding the insulin receptor tyrosine kinase inhibitor in proliferating liver and in liver tumors. Biochem. Biophys. Res. Commun. 180, 967-971; 1991.
- M. Tenan, B.M. Colombo, L. Cajola, B. Pollo, G. Broggi and G. Finocchiaro. Low frequency of NF1 gene mutations in malignant gliomas. Eur. J. Cancer 29, 1217-1218; 1993.
- M. Tenan, B.M. Colombo, B. Pollo, L. Cajola, G. Broggi and G. Finocchiaro. p53 mutations and microsatellite analysis of loss of heterozygosity in malignant gliomas. Cancer Genet. Cytogenet. 74, 139-143; 1994.
- I. Magnani, S. Guarneri, B. Pollo, N. Cirenei, B.M. Colombo, G. Broggi, C. Galli, O. Bugiani, S. DiDonato, G. Finocchiaro and A.M. Fuhrman Conti. Increasing complexity of the karyotype in 50 human gliomas: progressive evolution and de novo occurrence of cytogenetic alterations. Cancer Genet. Cytogenet. 75, 77-89; 1994.
- B.M. Colombo, R. Albarosa, L. Roz, I. Magnani, B. Pollo, N. Cirenei, C. Giani, A.M. Fuhrman Conti, S. DiDonato and G. Finocchiaro. Deletion mapping of gliomas suggests the presence of two small regions for candidate tumor-suppressor genes in a 17-cM interval on chromosome 10q. Am. J. Hum. Genet. 58, 1260-1267; 1996.
- I. Manni, N. Cirenei, R. Albarosa, B.M. Colombo, L. Roz, A. Sacchi, G. Piaggio and G. Finocchiaro. Mxi1 inhibits the proliferation of U-87 glioma cells through down-regulation of cyclin B1 gene expression. Br. J. Cancer 86, 477-484; 2002.
Gene therapy of cancer:
- B.M. Colombo, S. Benedetti, S. Ottolenghi, M. Mora, B. Pollo, G. Poli and G. Finocchiaro. The "bystander effect": association of U-87 cell death with ganciclovir-mediated apoptosis of nearby cells and lack of effect in athymic mice. Hum. Gene Ther. 6, 763-772; 1995.
- S. Benedetti, F. DiMeco, B. Pollo, N. Cirenei, B.M. Colombo, M.G. Bruzzone, E. Cattaneo, A. Vescovi, S. DiDonato, M.P. Colombo and G. Finocchiaro. Limited efficacy of the HSV-TK/GCV system for gene therapy of malignant gliomas and perspectives for the combined transduction of the interleukin-4 gene. Hum. Gene Ther. 8, 1345-1353; 1997.
- N. Cirenei, B.M. Colombo, M. Mesnil, S. Benedetti, H. Yamasaki and G. Finocchiaro. In vitro and in vivo effects of retrovirus-mediated transfer of the connexin 43 gene in malignant gliomas: consequences for HSV-tk/GCV anticancer gene therapy. Gene Therapy 5, 1221-1226; 1998.
Cancer and angiogenesis:
- M. Di Benedetto, A. Starzec, B.M. Colombo, D. Briane, G.Y. Perret, M. Kraemer and M. Crépin. Aponecrotic, antiangiogenic and antiproliferative effects of a novel dextran derivative on breast cancer growth in vitro and in vivo. Br. J. Pharmacol. 135, 1859-1871; 2002.
- B.M. Colombo, M. Sebbah-Louriki, D. El Manouni,, A. Martin, J.L. Salzmann, Y. Leroux, G.Y. Perret and M. Crépin. A new phenylacetate-bisphosphonate inhibits the in vitro and in vivo growth of breast cancer cells by proapoptotic and antiangiogenic effects. Anticancer Res. 22, 3925-3931; 2002.
- M. Benouchan, F. Do Nascimento, M. Sebbah-Louriki, J.L. Salzmann, M. Crépin, G.Y. Perret and B.M. Colombo. Bystander cell killing spreading from endothelial to tumor cells in a three-dimensional multicellular nodule model after E. coli nitroreductase gene delivery. Biochem. Biophys. Res. Commun. 311, 822-828; 2003.
- M. Benouchan, F. Do Nascimento, G.Y. Perret and B.M. Colombo. Delivery of bacterial nitroreductase gene into endothelial cells prolongs the survival of tumor-bearing mice by bystander mechanisms. Int. J. Oncol. 28, 457-462; 2006.
- M. Benouchan and B.M. Colombo. Anti-angiogenic strategies for cancer therapy: a review. Int. J. Oncol. 27, 563-571; 2005.
Cellular immunotherapy of cancer:
- C. Masurier, C. Pioche-Durieu, B.M. Colombo, R. Lacave, F.M. Lemoine, D. Klatzmann and M. Guigon. Immunophenotypical and functional heterogeneity of dendritic cells generated from murine bone marrow cultured with different cytokine combinations: implications for anti-tumoral cell therapy. Immunology 96, 569-577; 1999.
- B.M. Colombo, R. Lacave, C. Pioche-Durieu, C. Masurier, F.M. Lemoine, M. Guigon and D. Klatzmann. Cellular but not humoral immune responses generated by vaccination with dendritic cells protect mice against leukaemia. Immunology 99, 8-15; 2000.
- S. Paczesny, S. Beranger, J.L. Salzmann, D. Klatzmann and B.M. Colombo. Protection of mice against leukemia after vaccination with bone marrow-derived dendritic cells loaded with apoptotic leukemia cells. Cancer Res. 61, 2386-2389; 2001.
- R. Lacave, C. Masurier, M. Guigon, F.M. Lemoine, D. Klatzmann and B.M. Colombo. Similar immune responses and antitumor effects of murine dendritic cells loaded with either exogenous or endogenous-synthesized protein. Int. J. Oncology 23, 1747-1753; 2003.
Suppression of anticancer immune responses:
(work in progress, since 2007)
Other publications:
- S. Citterio, S. Sgorbati, M. Levi, B.M. Colombo and E. Sparvoli. PCNA and total nuclear protein content as markers of cell proliferation in pea tissue. J. Cell Sci. 102, 71-78; 1992.
- B.M. Colombo, D. Chiatante, S. Citterio and E. Sparvoli. Reactivation of cell proliferation in pea root meristems: presence and quantitative variations of a PCNA-like protein. Phytochem. (Life Sci. Adv.) 11, 213-217; 1992.
- B.M. Colombo, M. Peschanski. Clonage: en quoi sera-t-il thérapeutique? MT Médecine de la Reproduction, vol. 8, n. 4, juillet-août 2006.